The primary species targeted for conservation is the oncilla, or Leopardus pardinoides, whose population status is unknown because it is a recently described species. The only known fact is that its distribution areas have been restricted in Panama, and its habitat, the cloud forest ecosystem, has been impacted by feral and semi-feral dogs. This type of fauna transmits parasites and viral diseases that cause a decrease in their populations. Through six vaccination and deworming campaigns conducted from June to November, a total of 346 vaccines were administered, including rabies vaccines, achieving complete two-dose coverage for 173 free-roaming and semiferal dogs, as well as the treatment of 10 domestic cats. Dogs were vaccinated against rabies, canine distemper, adenovirus type 2, parainfluenza, parvovirus, and leptospirosis (Leptospira canicola and L. icterohaemorrhagiae), using live attenuated vaccines (Nobivac®). In addition, fenbendazole was administered as an antiparasitic treatment, dosed according to individual body weight, to reduce internal parasite loads relevant to wildlife and human health.With the support of licensed veterinarians, blood samples were collected from a subset of dogs for follow-up analyses of additional pathogens. These samples are currently under laboratory analysis and will contribute to a better understanding of disease dynamics at the wildlife–domestic animal interface. Several campaigns were conducted in collaboration with the Ministry of Health (MINSA), strengthening interinstitutional coordination and community trust in the implemented health actions.The project also strengthened scientific research, academic training, and national dissemination efforts. Under the ECOGRAFE Panamá project, student researchers Melany Pinzón and Edgar Gonzales actively participated in data collection and analysis on feral and semiferal fauna and their impacts on native wildlife. The results were presented at ENADES 2025 (National Sustainable Development Meeting), where the project received national recognition through the Rodrigo Tarté Ponce Award for Sustainability, highlighting its relevance to sustainable development and conservation.Additionally, community workshops on responsible pet ownership in wildlife areas were conducted, engaging local students and community members. These activities strengthened local awareness, encouraged community participation, and generated valuable data for ongoing research on feral and semiferal fauna and their impacts on native wildlife, with particular emphasis on small wild felids.