The goal of this investigation is to determine the actual occupancy estimation, habitat requirements and population health of the critically endangered Incilius holdridgei, Holdridge's toad. These results are then to be used for long term monitoring and planning for protection of the specie occurrence area.
1) Determine if Bd is present in Incilius holdridgei populations. This involves field monitoring and swab analysis to determine the presence or absence of Batrachochytrium dendrobatidis (Bd).
2) To determine the actual population size, extent of occurrence and area of occupancy of Holdrige´s toad.
3) Assess the habitat to identify requirements and potential risks through analysis of water, soil, vegetation and species co-occurrence.Methodology We shall visit localities monthly, from the beginning of the project to estimate the occupancy of I. holdridgei using occupancy models, environmental parameters will be taking. Each animal will be clinically examined by ensuring that they don’t have signs or symptoms of illness and/or external parasites and all animals will be sampled for Bd. Products
1. The presence of Bd in the analysis, this indicator is measured at the number of individuals who test positive in PCR analysis. 2. Occupancy estimation of species in the area studied 3. Estimate of area of presence 4. Identification of habitat requirements for I. holdridgei according with variables measured throught the study.
Project 13255863 location - Costa Rica, North America